My partner for this lab was Ariel. The purpose of the lab was to test how much oxygen was produced by the enzyme, which in this case was yeast.
The setup for this lab involve a graduated cylinder filled with water and submerged partly underwater upside down. It was submerged in a tub of water. Our reaction chamber was filled with 20 milliLiters of peroxide and plugged with a stopper with a hole in it. In the hole was a rubber tube that was placed in the opening of the graduated cylinder.
Once the set-up was complete, our testing began. We started with one milliLiter of the yeast solution and added it to the reaction chamber and quickly put the stopper in. The second and third tests were the same except for the amount of yeast solution. The second used .75 milliLiters and the third used .5 milliLiters.
Our results for the first test showed that the enzyme produced very little oxygen in the beginning. After recording every 30 seconds for 5 minutes, our results increased and the oxygen bubbles were larger and more frequent. It produced almost 15 milliLiters of oxygen in the end.
Our second and third test's results were similar, starting off slow but gradually increasing in the amount of oxygen. The second test produced a smaller amount of oxygen than the first test. The third test was similar in producing a smaller amount of oxygen than the first and second. However, the results showed that it produced hardly any oxygen at all and only created many very small bubbles instead of a few large bubbles like the other tests.
Based on these results, our conclusion is that the smaller amount of yeast in the solution, the less reaction it will have. It produces less oxygen with each decreasing amount. We believe that some inconsistencies may have been caused due to the amount of peroxide we put in the reaction chamber and the setup. Our very first trial had to be re-done because the stopper popped out of the chamber. Therefore, the results may be inconclusive, but the theory remains the same and valid.
Your post was well written. A couple things though, was yeast the enzyme or is the enzyme in the yeast? Also, in your explanation you should discuss that enzymes act as catalysts to increase reaction rate.
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